منابع مشابه
Glass beads load macromolecules into living cells.
We describe and characterize an exceptionally rapid and simple new technique for loading large numbers of cultured cells with large macromolecules. The culture medium of the cell monolayer is replaced by a small volume of the macromolecule to be loaded. Glass beads (75-500 micron diameter) are then sprinkled onto the cells, the cells are washed free of beads and exogenous macromolecules, and 'b...
متن کاملBiologically active catecholamines covalentyly bound to glass beads.
Catecholamines bound covalently to glass beads have been found to have biological activity in several systems. Experimental evidence has been found that immobilized epinephrine and isoproterenol accelerate the heart rate in dogs, chick embryo, and chick heart cells grown in culture, whereas immobilized propranolol results in a decrease in heart rate. Isoproterenol bound to glass beads has also ...
متن کاملTransformation of yeast by agitation with glass beads.
We have found that agitation of Saccharomyces cerevisiae with glass beads and plasmid DNA using a vortex mixer results in genetic transformation of the yeast cells. This method is less efficient, but considerably more convenient, than other yeast transformation procedures. The fact that the minimal requirements for transformation are simply physical damage and the presence of DNA in an osmotica...
متن کاملSurface Modification of Glass Beads with an Aminosilane Monolayer
Self-assembled monolayers (SAMs) are monomolecular layers which are spontaneously formed upon immersing a solid substrate into a solution containing functional molecules. The most attractive feature of SAMs is molecular level control over the modification of surfaces, and incorporation of multiple or multilayer molecular components onto the monolayer leads to various functional properties. SAMs...
متن کاملFacile and Rapid Formation of Giant Vesicles from Glass Beads
1 Department of Chemistry, University of Fribourg, Chemin du Musée 9, 1700 Fribourg, Switzerland; [email protected] (R.T.); [email protected] (U.M.) 2 Department of Biochemistry, University of Geneva, 30, Quai Ernest-Ansermet, 1211 Geneva, Switzerland; [email protected] (A.C.); [email protected] (A.R.) 3 National Centre of Competence in Research in Chemical Biology, 1211 Geneva, ...
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ژورنال
عنوان ژورنال: Internet Archaeology
سال: 2015
ISSN: 1363-5387
DOI: 10.11141/ia.40.1.worrell